Bioprepare Microbiology

BIOCHROM LACTOBACILLUS AGAR

BIOCHROM LACTOBACILLUS AGAR is a chromogenic plate used for the cultivation, isolation, enumeration and differentiation of Lactobacilli from clinical specimens and foods. This medium allows the distinction between Lactobacilli that produce hydrogen peroxide (H₂O₂), which appear with blue colonies, and Lactobacilli that do not produce H₂O₂, which appear with white colonies.

The modification of LACTOBACILLUS SELECTIVE AGAR with Chromogenic mix allows the visual differentiation of Lactobacilli:

  • Lactobacilli that produce H₂O₂ oxidize the Chromogenic mix, causing blue staining of the colonies.
  • Lactobacilli that do not produce H₂O₂ remain white, as they lack the necessary enzymatic activity for oxidation.

This differentiation is important in diagnostic tests, as H₂O₂-positive Lactobacilli protect vaginal and intestinal health. In food microbiology, it helps monitor the microbial composition of dairy products.

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